（一）-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响

doi:10.11843/j.issn.0366-6964.2017.05.020

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (5): 945-953.doi: 10.11843/j.issn.0366-6964.2017.05.020

（一）-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响

李龙龙, 彭梦玲, 王典, 俞磊, 葛重阳, 马海田^*

南京农业大学动物医学院农业部动物生理生化重点实验室, 南京 210095

收稿日期:2016-11-07 出版日期:2017-05-23 发布日期:2017-05-23
通讯作者: 马海田(1974-),教授,E-mail:mahaitian@njau.edu.cn
作者简介:李龙龙(1991-),男,安徽蚌埠人,硕士生,主要从事动物机能生物化学研究,E-mail:2014107013@njau.edu.cn
基金资助:
国家自然科学基金（31572483）；江苏省自然科学基金（BK20151434）

The Effect of (一)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes

LI Long-long, PENG Meng-ling, WANG Dian, YU Lei, GE Chong-yang, MA Hai-tian^*

Key Laboratory of Animal Physiology and Biochemistry of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China

Received:2016-11-07 Online:2017-05-23 Published:2017-05-23

摘要/Abstract

摘要：

试验旨在研究（一）-HCA对鸡胚原代肝细胞增殖和线粒体超微结构及功能的影响。笔者选用终浓度为0、1、10和50 μmol·L^-1的（一）-HCA处理鸡胚原代肝细胞后收集细胞，检测细胞增殖及线粒体功能相关指标。结果表明，（一）-HCA处理可促进鸡胚原代肝细胞增殖，10 μmol·L^-1（一）-HCA处理可极显著升高S期和G2/M期细胞比例（P<0.01），并提高周期相关因子的mRNA转录。1~50 μmol·L^-1（一）-HCA处理对鸡胚原代肝细胞线粒体形态、数量和膜电位均无显著影响（P>0.05），但（一）-HCA处理可显著升高原代鸡胚肝细胞线粒体中柠檬酸合酶和琥珀酸脱氢酶活性（P<0.05）。结果提示，（一）-HCA处理可通过调节周期相关因子的表达而促进鸡胚原代肝细胞的增殖，同时其可通过加速三羧酸循环进而增强细胞的能量代谢水平。

Abstract:

The present study aimed to investigate the effects of (一)-Hydroxycitric acid (HCA) on cells proliferation, mitochondrial ultrastructure and function in primary chicken hepatocytes. The cells were treated with 0, 1, 10 and 50 μmol·L^-1 (一)-HCA and then detected the factors related with cells proliferation and mitochondria function. The results showed that (一)-HCA treatment promoted the cell proliferation in primary chicken hepatocytes. Ten μmol·L^-1 (一)-HCA treatment significantly increased the S phase and G2/M phase cell population (P<0.01), meanwhile it increased the cyclin mRNA expression level when compared to control group. No significantly changes were observed on the mitochondrial morphology, quantity and membrane potential in primary chicken hepatocytes following 1-50 μmol·L^-1 (一)-HCA treatment (P>0.05), while (一)-HCA treatment significantly increased the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes (P<0.05). These results indicated that (一)-HCA treatment promote the primary chicken hepatocytes proliferation by increasing cyclin mRNA expression, whereas it increase the energy metabolism by enhancing the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes.

中图分类号:

S852.23

李龙龙, 彭梦玲, 王典, 俞磊, 葛重阳, 马海田. （一）-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响[J]. 畜牧兽医学报, 2017, 48(5): 945-953.

LI Long-long, PENG Meng-ling, WANG Dian, YU Lei, GE Chong-yang, MA Hai-tian. The Effect of (一)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 945-953.

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（一）-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响

The Effect of (一)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes

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